This algorithm first determines the portion of the amplification plots where all of the data curves display an exponential increase in fluorescence.
To do this, the software looks at the shift in fluorescence for each baseline- corrected curve and sets a point just above the baseline at 0% and the maximum of the first derivative as 100% amplification.
As a default, the search range for the algorithm falls within 5–60% of this luorescence shift for all the curves.
This range can be manually adjusted, based on personal preferences, by accessing the Analysis Terms Settings Screen (on the menu bar under Options). Under the Threshold Fluorescence tab, select the Advanced Settings button to enter the new range.